[Identification and metabolism characterization of a Clostridium lituseburense strain isolated from high-altitude
Quanquan Liu1, Haichang Liu, Yu Deng
1Biogas Institute of Ministry of Agriculture, Key Laboratory of Energy Microbiology and Application, Chengdu 610041, China. luckilylq@yahoo.com.cn
Objective:
We studied the physiological, biochemical properties and metabolism of Clostridium lituseburense P4-1 from soil in Namucuo.
Methods:
We adopted Hungate anaerobic technique to get Strain P4-1 from soil in Namucuo. Through physiological, biochemical and phylogenetic analysis, we identified the strain P4-1.
Results:
Cells were Gram-positive and spore-forming. It grew between 13 and 40 degrees C (optimum at 37 degrees C), between pH value 5.0 and 10.O (optimum at 7.5-8.0), and with the presence of NaCl between 0%-5%. Strain P4-1 could metabolize many carbon sources including glucose, melibiose and mannitol. Metabolites of glucose were acetate, butyrate, propionate, CO2, and little H2. Based on 16S rDNA studies, strain P4-1 was most close to Clostridium lituseburense DSM 797 (M59107) with 98.7% similarity. Strain P4-1 could degrade p-toluene sulfonate.
Conclusion:
Strain P4-1 tolerated low temperature, salt and could degrade p-toluene sulfonate. Its metabolites produced by fermentation of glucose could improve the soil micro-environment. It was significant for strain P4-1 to be utilized in the wastewater treatment.
