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Updated: Jun 6, 2026
Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris
Published on: February 25, 2010
[Expression and bioactivity assay of mature chicken interleukin-18 protein mutant using Pichia pastoris expression
Xihui Wang1, Shousong Yue, Jingdong Hu
1College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Shandong Taian 271018, China. wangxi_hui@163.com
Objective:
We expressed mature chicken interleukin-18 (mChIL-18) in Pichia pastoris.
Methods:
The mChIL-18 gen was reconstructed by using site-specific mutagenesis based on the Pichia pastoris-preferred codons. The recombinant plasmid pPIC9K/mChIL-18 was constructed and transformed to Pichia pastoris GS115 by electroperation. Multi-copy recombinant strains were screened by Geneticin (G418). The expression of mChIL-18 protein was induced by methanol. SDS-PAGE and Western-blot were used to analyze the expressed products. The bioactivity of mChIL-18 was measured by methyl thiazolyl tetrazolium assays and chicken embryo fibroblasts-vesicular stomatitis virus (CEF-VSV) system.
Results:
The protein of mChIL-18 could be secreted by GS115. The optimum expression conditions, a rate of 480 mg/L,were obtained as follows:temperature 28 degrees C, pH 6.5, methanol concentration 2% and expression time 120 h. The obtained mChIL-18 protein could stimulate T lymphocytes proliferation. IFN-gamma induced by mChIL-18 could directly inhibit the growth of VSV in CEF,and its antiviral activity was about 1.7 x 10(4) U/mL which was produced by 400 microg/L of mChIL-18.
Conclusion:
The high expression of bioactive recombinant mature chicken interleukin-18 (mChiL-18) in Pichia pastoris had been achieved.
