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A direct radioimmunoassay for tonin.
Canadian Journal of Biochemistry
|August 1, 1978
Summary
A new radioimmunoassay accurately measures tonin, a key enzyme, in biological samples. This method allows for precise quantification of tonin levels in rat saliva and submaxillary glands.
Area of Science:
- Biochemistry
- Enzymology
- Assay Development
Background:
- Tonin is an enzyme involved in blood pressure regulation.
- Accurate measurement of tonin is crucial for physiological studies.
- Existing methods for tonin quantification have limitations.
Purpose of the Study:
- To develop and validate a sensitive radioimmunoassay for measuring tonin.
- To apply the assay for quantifying tonin in rat biological samples.
Main Methods:
- Production of rabbit antibodies against purified rat submaxillary gland tonin.
- Radioiodination of tonin using the chloramine-T method.
- Optimization of radioimmunoprecipitation and use of the double-antibody method for separation.
Main Results:
- The developed radioimmunoassay is highly sensitive, detecting as low as 150 pg of tonin.
- The assay demonstrated excellent correlation with fluorometric measurements of tonin activity.
- Successful application of the assay to measure tonin in rat saliva and submaxillary gland homogenates.
Conclusions:
- A sensitive and reliable radioimmunoassay for tonin measurement has been established.
- This assay provides a valuable tool for research in the renin-angiotensin system.
- The method allows for precise quantification of tonin in various biological matrices.