Effects of iRoot SP on mineralization-related genes expression in MG63 cells
1Department of Operative Dentistry and Endodontics, School of Stomatology, Wuhan University,Wuhan, China.
Introduction:
The purpose of this study was to assess the effects of iRoot SP root canal sealer (Innovative BioCreamix Inc, Vancouver, Canada) on the expression of mineralization-related genes in human MG63 osteoblast-like cells.
Methods:
Specimens (5 mm in diameter and 2 mm in height) of iRoot SP and AH Plus (Dentsply DeTrey, Konstanz, Germany) were extracted from a 5-mL culture medium. The MG63 cells were exposed to various dilutions (1/1, 1/2, and 1/4) of the extracts. The 3,(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide MTT assay was used for assessing the dental materials' nonspecific cytotoxicity. The expression of mineralization-related genes, including collagen type I (COL I), osteocalcin (OCN), bone sialoprotein (BSP) and osteopontin (OPN), was detected on days 1, 3, and 6 by a real-time polymerase chain reaction. An enzyme-linked immunosorbent assay experiment was used for evaluating COL I and BSP protein changes. The data were analyzed with one-way analysis of variance and Tukey tests.
Results:
In the MTT assay, the undiluted extracts of iRoot SP were noncytotoxic, whereas the undiluted extracts of AH Plus were rated as slightly cytotoxic. iRoot SP up-regulated COL I, OCN, and BSP messenger RNA expression after 3 and 6 days. In the enzyme-linked immunosorbent assay experiment, iRoot SP increased COL I and BSP protein levels compared with AH Plus and the control group on day 6.
Conclusions:
In the presence of iRoot SP, MG63 cells can produce more mineralized matrix gene and protein expression. Based on these results, iRoot SP can be considered as a favorable material for cell-material interaction.
