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Methods to Investigate the Regulatory Role of Small RNAs and Ribosomal Occupancy of Plasmodium falciparum
Published on: December 4, 2015
Plasmodium falciparum spliceosomal RNAs: 3' and 5' end processing
Calvo Eliana1, Escobar Javier, Wasserman Moisés
1Universidad Nacional de Colombia, Facultad de Ciencias, Laboratorio de Investigaciones Básicas en Bioquímica, Bogotá Colombia.
Abstract:
The major spliceosomal small nuclear ribonucleoproteins (snRNPs) consist of snRNA (U1, U2, U4 or U5) and several proteins which can be unique or common to each snRNP particle. The common proteins are known as Sm proteins; they are crucial for RNP assembly and nuclear import of spliceosomal RNPs. This paper reports detecting the interaction between Plasmodium falciparum snRNAs and Sm proteins, and the usual 5' trimethylated caps on the snRNAs, by immunoprecipitation with specific antibodies. Furthermore, an unusual poly(A) tail was detected on these non-coding RNAs.
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