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Updated: Jun 6, 2026
MicroRNA Based Liquid Biopsy: The Experience of the Plasma miRNA Signature Classifier (MSC) for Lung Cancer Screening
Published on: October 26, 2017
Cell-free miR-24 and miR-30d, potential diagnostic biomarkers in malignant effusions
Li Xie1, Tingting Wang, Shaorong Yu
1The Comprehensive Cancer Center of Drum Tower Hospital affiliated to Medical School of Nanjing University & Clinical Cancer Institute of Nanjing University, Nanjng 21008, PR China; Jiangsu Key Laboratory for Molecular Medicine, Nanjing 210008, PR China.
Objectives:
The study was trying to figure out the possible role of cell-free miR-24 and miR-30d as diagnostic biomarkers for malignant effusions.
Methods:
A total of 110 effusion samples (28 benign and 82 malignant) were collected and cell-free total RNA was extracted. Quantitative RT-PCR was carried out to measure the expression levels of miR-24 and miR-30d, with an exogenous supplemented plant miRNA, ath-miR156a as the reference RNA.
Results:
Malignant pleural effusions had higher expression levels of cell-free miR-24 and miR-30d than benign effusions. In the ascites group, only miR-30d was found to be significantly up-regulated in the supernatant of effusions. Combination of cell-free miR-24 and miR-30d could discriminate malignant ascites from benign with improved potency comparing to single miR-30d.
Conclusions:
Cell-free miR-30d and miR-24 are potential diagnostic biomarkers for malignant and benign effusions.
