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Related Concept Videos

DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
DNA Isolation01:34

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.
Immunoprecipitation01:20

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...
Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

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Electricity-Free, Sequential Nucleic Acid and Protein Isolation
09:52

Electricity-Free, Sequential Nucleic Acid and Protein Isolation

Published on: May 15, 2012

Improved method for simultaneous isolation of proteins and nucleic acids.

Soroth Chey1, Claudia Claus, Uwe Gerd Liebert

  • 1Institute of Virology, University of Leipzig, Leipzig, Germany.

Analytical Biochemistry
|November 25, 2010
PubMed
Summary

This study introduces a faster, more efficient method for protein isolation using ethanol-bromochloropropane-water precipitation after guanidinium thiocyanate-phenol-chloroform extraction. This technique improves protein solubility for subsequent analysis, aiding molecular biology research.

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Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genetics

Background:

  • Guanidinium thiocyanate-phenol-chloroform (GTPC) extraction is standard for nucleic acid and protein isolation.
  • Commercial GTPC methods for protein isolation are slow and yield poorly soluble pellets.

Purpose of the Study:

  • To develop a rapid and efficient protein isolation protocol.
  • To improve protein solubility for downstream analysis following GTPC extraction.

Main Methods:

  • Utilized ethanol-bromochloropropane-water for protein precipitation from the phenol-ethanol phase after GTPC RNA/DNA extraction.
  • Dissolved precipitated proteins in 4% SDS for analysis.

Main Results:

  • Achieved protein precipitation and ready dissolution in 4% SDS.
  • Reduced protein isolation time to 30 minutes.
  • Obtained high protein recovery rates of up to 95%.

Conclusions:

  • The new method offers a fast and efficient alternative for protein extraction.
  • Enables simultaneous analysis of transcriptional and posttranscriptional events from a single sample.