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Updated: Jun 6, 2026

Evaluation of Antimicrobial Activities of Nanoparticles and Nanostructured Surfaces In Vitro
Published on: April 21, 2023
Ag colloids and Ag clusters over EDAPTMS-coated silica nanoparticles: synthesis, characterization, and antibacterial
Shiva K Rastogi1, Veronica J Rutledge, CharLene Gibson
1Biosensors and Nanotechnology Application Laboratory (BNAL), University of Idaho, Coeur D'Alene, Coeur d'Alene, Idaho, USA. srastogi@uidaho.edu
Abstract:
To produce better antibacterial water-insoluble nanocomposites of silver (Ag), silver-silicon dioxide (Ag-SiO(2)) hybrid and silver colloid (Ag-c) nanoparticles (NPs) were studied. Ag-c NPs were synthesized using reduction of AgNO(3), and Ag-SiO(2) composites were prepared on a core of silica NPs functionalized with ethylenediamino-propyltrimethoxysilane, where Ag clusters were fabricated on amino groups using seed-mediated growth and characterized by transmission electron microscopy and ultraviolet-visible absorption spectroscopy. Antibacterial effectiveness of the Ag-SiO(2) NPs was tested against general Escherichia coli (E. coli ATCC 25922) and E. coli O157:H7 by measuring the growth based on optical density and digital counting of live-dead cells using a fluorescent microscope, and a field emission scanning electron microscope. Minimum inhibitory concentration values were studied against four representative bacteria along with E. coli O157:H7. Results showed that Ag NPs of 6.6 ± 4.5 nm were attached to the surface of SiO(2) NPs (74 ± 13.5 nm), and the Ag-c NPs (3.5 ± 2 nm) showed excellent antibacterial properties.
From The Clinical Editor:
In this paper, the synthesis of Ag colloids and Ag clusters over EDAPTMS-coated silica nanoparticles is reported. Both NPs were examined for antibacterial effectiveness against representative bacteria including E. coli O157:H7 and found to have excellent antibacterial properties.
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