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Bovine T-lymphocyte lines reactive with Brucella abortus.
R Smith1, J C Kapatsa, B A Rosenbaum
1Department of Veterinary Pathology, College of Veterinary Medicine, Texas A&M University, College Station 77843-4463.
American Journal of Veterinary Research
|April 1, 1990
Summary
Researchers established bovine T-cell lines reactive to Brucella abortus. These cell lines, crucial for understanding bovine immune responses, identified CD4+ T-cells and a novel T-lymphocyte subset.
Area of Science:
- Immunology
- Veterinary Science
- Cell Biology
Background:
- Brucella abortus is a significant pathogen in cattle, causing brucellosis.
- Understanding bovine T-cell responses is critical for developing effective vaccines and treatments.
- Previous research has limited characterization of long-term, antigen-specific bovine T-cell lines.
Purpose of the Study:
- To establish and characterize long-term bovine T-cell lines reactive to Brucella abortus.
- To investigate the phenotype and functional requirements of these T-cell lines.
- To identify potential T-lymphocyte subsets involved in the bovine immune response to Brucella.
Main Methods:
- Establishment of T-cell lines through repeated stimulation with B. abortus and antigen-presenting cells.
- Thymidine-incorporation assays to measure cellular proliferation in response to Brucella strains.
- Flow cytometry to analyze cell surface markers, including CD4, CD8, and CD2.
- Assessment of antigen-presenting cell requirements for T-cell activation.
Main Results:
- Successfully established 33 antigen-specific bovine T-cell lines from 14 cows.
- T-cell lines responded to virulent, vaccine, and rough mutant Brucella strains.
- Antigen-presenting cells were required for T-cell response, with autologous cells being optimal.
- All cell lines expressed CD4+ T-cell markers; none expressed the bovine CD8 equivalent.
- A subset of T-lymphocytes with a CD2+CD4-CD8- phenotype was identified, potentially representing a novel T-cell subset.
Conclusions:
- Long-term bovine T-cell lines reactive to Brucella abortus can be successfully established.
- These cell lines represent key components of the bovine adaptive immune response to Brucella.
- The identified T-cell subsets, including CD4+ and a CD2+CD4-CD8- population, offer insights into cellular immunity against Brucella infections in cattle.