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Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Long-patch Base Excision Repair01:02

Long-patch Base Excision Repair

Since the discovery of the two BER pathways, there has been a debate about how a cell chooses one pathway over the other and the factors determining this selection. Numerous in vitro experiments have pointed out multiple determinants for the sub-pathway selection. These are:
Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...

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Related Experiment Video

Updated: Jun 6, 2026

Low-cost Custom Fabrication and Mode-locked Operation of an All-normal-dispersion Femtosecond Fiber Laser for Multiphoton Microscopy
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Low-cost Custom Fabrication and Mode-locked Operation of an All-normal-dispersion Femtosecond Fiber Laser for Multiphoton Microscopy

Published on: November 22, 2019

A pause to splice.

Pia K Andersen1, Torben Heick Jensen

  • 1Department of Molecular Biology, Centre for mRNP Biogenesis and Metabolism, CF Møllers Allé 3, Building 1130, Aarhus University, 8000 Aarhus, Denmark.

Molecular Cell
|November 25, 2010
PubMed
Summary
This summary is machine-generated.

Messenger RNA (mRNA) processing is crucial for gene expression. New research shows that RNA polymerase II (RNAPII) pausing aids both mRNA capping and cotranscriptional splicing in yeast.

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Complementation of Splicing Activity by a Galectin-3 - U1 snRNP Complex on Beads
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Related Experiment Videos

Last Updated: Jun 6, 2026

Low-cost Custom Fabrication and Mode-locked Operation of an All-normal-dispersion Femtosecond Fiber Laser for Multiphoton Microscopy
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Complementation of Splicing Activity by a Galectin-3 - U1 snRNP Complex on Beads
08:48

Complementation of Splicing Activity by a Galectin-3 - U1 snRNP Complex on Beads

Published on: December 9, 2020

Area of Science:

  • Molecular Biology
  • Gene Expression
  • RNA Processing

Background:

  • mRNA termini maturation, including capping, is essential for gene expression.
  • RNA polymerase II (RNAPII) pausing has been previously linked to facilitating mRNA capping.
  • The precise mechanisms regulating cotranscriptional RNA processing remain an active area of research.

Discussion:

  • This study investigates the role of RNAPII pausing in cotranscriptional splicing.
  • Two independent groups provide evidence for RNAPII pausing's involvement in splicing in S. cerevisiae.
  • The findings suggest a coordinated regulation of mRNA processing events during transcription.

Key Insights:

  • RNAPII pausing is not only involved in mRNA capping but also significantly assists cotranscriptional splicing.
  • This highlights a dual role for RNAPII pausing in ensuring the fidelity and efficiency of mRNA maturation.
  • The research deepens our understanding of the intricate coupling between transcription and RNA processing.

Outlook:

  • Further investigation into the specific pausing elements and their interaction with splicing machinery is warranted.
  • Exploring whether this phenomenon is conserved across different organisms and cell types.
  • Potential implications for understanding splicing-related human diseases.