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Updated: Oct 17, 2026

Combined Nucleotide and Protein Extractions in Caenorhabditis elegans
Published on: March 17, 2019
A unique mRNA species for the alpha subunit of Gs is present in rat haploid germ cells
T B Haugen1, E J Paulssen, J O Gordeladze
1Institute of Medical Biochemistry, University of Oslo, Norway.
Abstract:
The 1.9 kb mRNA encoding the alpha subunit of Gs observed in the somatic cells of the rat testis was not seen in the germ cells (pachytene spermatocytes and round spermatids) as revealed by Northern blot analysis. Interestingly, the cDNA probe for Gs alpha detected a mRNA of shorter chain length (0.9 kb) exclusively in the haploid germ cells (round and elongated spermatids). However, no ADP ribosylated protein was detected in membranes from germ cells after cholera toxin treatment. The 0.9 kb transcript may represent a haploid germ cell-specific variant of Gs alpha, which is functionally different from the stimulatory component of the adenylate cyclase system.
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