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Updated: Jun 6, 2026
11:12
Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material
Published on: August 1, 2018
Altered antisense-to-sense transcript ratios in breast cancer
Reo Maruyama1, Michail Shipitsin, Sibgat Choudhury
1Department of Medical Oncology, and Belfer Institute for Applied Cancer Science, Dana-Farber Cancer Institute, Boston, MA 02115, USA.
Summary
Researchers found significant differences in RNA transcript distribution between normal and cancerous breast tissues. These changes in noncoding RNAs (ncRNAs) suggest distinct RNA regulation in cancer, potentially contributing to tumor development.
Area of Science:
- Genomics
- Molecular Biology
- Cancer Research
Background:
- Emerging evidence highlights the role of noncoding RNAs (ncRNAs) in physiological processes and cancer.
- Transcriptome profiling reveals extensive genomic transcription beyond protein-coding genes.
Purpose of the Study:
- To investigate differences in sense and antisense transcript distribution between normal and neoplastic breast tissues.
- To explore the potential role of ncRNAs in tumorigenesis.
Main Methods:
- Comparative transcriptome profiling of normal and breast cancer tissues.
- Analysis of sense and antisense transcript distribution patterns.
Main Results:
- Consistent and significant differences in transcript distribution were observed between normal and neoplastic breast tissues.
- Many differentially expressed antisense transcripts were identified as long noncoding RNAs (lncRNAs).
- A subset of genes primarily producing antisense transcripts in normal cells, but not cancer cells, are involved in critical metabolic processes.
Conclusions:
- Fundamental differences in global RNA regulation exist between normal and cancer cells.
- Altered ncRNA expression patterns may contribute to the development of breast cancer.
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