Targeting antibodies to the cytoplasm
Andrea L J Marschall1, André Frenzel, Thomas Schirrmann
1Technische Universität Braunschweig; Institute of Biochemistry and Biotechnology; Braunschweig, Germany.
Mabs
|November 25, 2010
Summary
Researchers are developing intracellular antibodies for cell imaging and functional studies. This review covers technologies for expressing these antibody fragments within living cells, overcoming challenges of membrane transport for improved therapeutic applications.
Area of Science:
- Biotechnology
- Molecular Biology
- Cellular Biology
Background:
- Research consortia are generating antibodies and fragments targeting the human proteome.
- Intracellular applications like imaging and functional intervention are highly valuable.
- Traditional antibodies require membrane translocation, posing delivery challenges.
Purpose of the Study:
- To review technologies for targeting intracellular proteins with antibodies.
- To highlight methods enabling intracellular expression of antibody fragments.
- To discuss applications in cellular imaging and functional intervention.
Main Methods:
- Review of existing literature and technologies.
- Analysis of phage display systems for antibody gene availability.
- Discussion of methods for intracellular antibody expression.
Main Results:
- Phage display systems facilitate intracellular expression of antibody genes.
- Various technologies exist to enable antibody targeting within cells.
- Intracellular expression bypasses the need for membrane transport.
Conclusions:
- Intracellular antibody expression offers a promising avenue for cellular research and therapeutics.
- Advances in antibody engineering and expression systems are crucial.
- Targeting intracellular proteins with antibodies has significant potential.
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