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Related Concept Videos

Hydrolysis01:15

Hydrolysis

Overview
Hydrolysis is a chemical reaction in which the addition of water breaks down a polymer into its simpler monomer units. For example, peptides break into amino acids, carbohydrates into simple sugars, and DNA into nucleotides. Enzymes often facilitate these processes.
Hydrolysis Reverses Dehydration Synthesis
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Inborn Errors of Metabolism01:20

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Phenylketonuria (PKU) is a protein metabolism disorder characterized by high blood levels of the amino acid phenylalanine. This results from a mutation in the gene responsible for phenylalanine hydroxylase, an enzyme that converts phenylalanine into tyrosine. When this enzyme is deficient, phenylalanine builds up in the blood, leading to symptoms such as vomiting, rashes, seizures, growth deficiency, and severe mental retardation. An early diagnosis and a diet restricting phenylalanine intake...
Inducible Operons: lac Operon01:25

Inducible Operons: lac Operon

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Updated: Jul 8, 2026

Measuring Lactase Enzymatic Activity in the Teaching Lab
04:41

Measuring Lactase Enzymatic Activity in the Teaching Lab

Published on: August 6, 2018

Lactose intolerance.

H A Büller1, R J Grand

  • 1Department of Pediatrics, Academic Medical Center, Amsterdam, The Netherlands.

Annual Review of Medicine
|January 1, 1990
PubMed
Summary
This summary is machine-generated.

Lactose intolerance, a common issue, stems from low lactase levels due to intestinal damage or genetic factors affecting lactase-phlorizin hydrolase. Research is advancing the understanding of this enzyme and related clinical problems.

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Last Updated: Jul 8, 2026

Measuring Lactase Enzymatic Activity in the Teaching Lab
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Published on: August 6, 2018

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Area of Science:

  • Biochemistry
  • Gastroenterology
  • Genetics

Background:

  • Lactose intolerance is a widespread clinical condition.
  • It is characterized by insufficient lactase enzyme activity.
  • This deficiency can arise from intestinal injury or genetic downregulation of lactase-phlorizin hydrolase.

Purpose of the Study:

  • To review the current understanding of lactose intolerance.
  • To highlight the molecular and cellular biology of lactase-phlorizin hydrolase.
  • To discuss the scientific basis of clinical syndromes associated with low lactase activity.

Main Methods:

  • Literature review of molecular and cellular biology.
  • Analysis of genetic expression patterns of lactase-phlorizin hydrolase.
  • Synthesis of clinical data related to lactase deficiency.

Main Results:

  • Low lactase levels are a primary cause of lactose intolerance.
  • Genetic alterations in lactase-phlorizin hydrolase expression are common in adults globally.
  • Progress is being made in understanding the enzyme's biology and associated clinical issues.

Conclusions:

  • Lactose intolerance has diverse etiologies, including genetic and injury-related factors.
  • Further research into lactase-phlorizin hydrolase is crucial for clinical advancements.
  • Improved understanding aids in managing lactose intolerance and related gastrointestinal disorders.