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Maternal effect on micronucleus induction in MS/Ae mice
Abstract:
MS/Ae mice, which are mutagen-sensitive in both the dominant lethal test and micronucleus test, and CD-1 mice, which are the parental strain of MS/Ae, were mated in all four possible combinations. Both male and female offspring were subjected to the micronucleus test using mitomycin C (MMC), colchicine (Col), and 6-mercaptopurine (6-MP). Col showed equivocal results. However, MMC and 6-MP showed differential responses in that both male and female offspring from CD-1 dams had lower incidences of micronucleated polychromatic erythrocytes than those from MS/Ae dams regardless of sire strain. In addition, body weights of offspring from MS/Ae dams were lower than those from CD-1 dams regardless of sire strain. Numbers of offspring from MS/Ae dams tended to be smaller than those from CD dams. These results suggest that the traits of MS/Ae mice are associated more with maternal factors than with paternal ones.
Insights
Maternal factors in MS/Ae mice influence mutagen sensitivity and offspring development. These mutagen-sensitive mice showed lower incidences of micronucleated cells and reduced body weight when offspring were maternally derived from MS/Ae dams.
Area of Science:
- Genetics
- Toxicology
- Developmental Biology
Background:
- MS/Ae mice exhibit mutagen sensitivity in dominant lethal and micronucleus tests.
- CD-1 mice are the parental strain for MS/Ae mice.
- Understanding genetic and maternal influences on mutagen sensitivity is crucial.
Purpose of the Study:
- To investigate the influence of maternal versus paternal factors on mutagen sensitivity in MS/Ae mice.
- To assess differential responses to mutagens in offspring from reciprocal crosses.
Main Methods:
- Reciprocal crosses between MS/Ae and CD-1 mice.
- Micronucleus test on male and female offspring using mitomycin C (MMC), colchicine (Col), and 6-mercaptopurine (6-MP).
- Evaluation of micronucleated polychromatic erythrocyte incidence and offspring body weight.
Main Results:
- Mitomycin C and 6-mercaptopurine showed differential responses.
- Offspring from CD-1 dams had lower micronucleated cell incidences than those from MS/Ae dams.
- Offspring from MS/Ae dams exhibited lower body weights and tended to have smaller litter sizes.
Conclusions:
- Mutagen sensitivity traits in MS/Ae mice are predominantly associated with maternal factors.
- Maternal genetic or environmental influences play a significant role in offspring mutagen response and development.
- Further research is needed to elucidate specific maternal mechanisms involved.