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Rapid purification of the peptide toxins microcystin-LR and nodularin
C Martin1, K Sivonen, U Matern
1Institut für Biologie II, Mikrobiologie, Albert-Ludwigs-Universität, Freiburg i. Br., F.R.G.
FEMS Microbiology Letters
|March 1, 1990
Abstract:
An isolation procedure was developed to provide within one day microcystin-LR, a cyclic heptapeptide toxin from Microcystis aeruginosa PCC 7806. After ODS (octadecylsilyl) solid phase extraction, the crude toxin fraction was chromatographed using a strong anion exchange column. The toxin was eluted with 0.02 M ammonium bicarbonate. An at least 95% purity was revealed on HPLC separation by monitoring at 214 nm. Application of the procedure to the cyclic pentapeptide toxin nodularin from Nodularia spumigena AV2 was examined.