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Related Experiment Videos

Sequence similarities among monkey ori-enriched (ors) fragments.

B S Rao1, M Zannis-Hadjopoulos, G B Price

  • 1Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD 20892.

Gene
|March 15, 1990
PubMed
Summary

Monkey DNA fragments, enriched for origins of replication (ori), were analyzed. Seven of eight fragments contained conserved sequences, suggesting potential roles in DNA replication initiation.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Origins of DNA replication (ori) are crucial for cell division.
  • Previous studies suggested some monkey DNA fragments could function as oris.
  • The 'O-family' of repetitive sequences in human DNA shares similarities with transposon-like elements.

Purpose of the Study:

  • To determine the nucleotide sequences of eight ori-enriched fragments from monkey DNA.
  • To investigate potential functional similarities with known DNA replication origins.
  • To identify conserved sequence elements within these ori fragments.

Main Methods:

  • Isolation of ori-enriched DNA fragments from monkey DNA.
  • Nucleotide sequencing of the isolated fragments.

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  • Sequence comparison with known consensus sequences (Dierks, Gasser/Laemmli, yeast ARS).

    • Main Results:

    • Eight ori-enriched fragments were sequenced.
    • No significant common sequences were found, except for A+T-rich stretches.
    • Seven of eight fragments contained conserved sequences matching Dierks, Gasser/Laemmli, and yeast ARS consensus sequences.

    Conclusions:

    • The identified monkey DNA fragments share conserved sequences with known DNA replication origins.
    • These conserved sequences may play a role in DNA replication initiation in vivo.
    • The findings contribute to understanding the structural requirements for origins of DNA replication.