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Related Experiment Videos

A transferable plasmid associated with AS-48 production in Enterococcus faecalis.

M Martínez-Bueno1, A Gálvez, E Valdivia

  • 1Departamento de Microbiología, Facultad de Ciencias, Universidad de Granada, Spain.

Journal of Bacteriology
|May 1, 1990
PubMed
Summary

Enterococcus faecalis S-48 produces the antibiotic AS-48. A 56-kilobase plasmid (pMB2) was identified as potentially carrying the AS-48 genes, confirmed by successful transfer and production in a new strain.

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Area of Science:

  • Microbiology
  • Bacteriology
  • Molecular Biology

Background:

  • Enterococcus faecalis S-48 is known to produce the peptide antibiotic AS-48 and a bacteriocin, Bc-48.
  • Investigating the genetic basis of AS-48 production is crucial for understanding its biosynthesis and potential applications.

Purpose of the Study:

  • To identify the genetic elements responsible for AS-48 production in Enterococcus faecalis S-48.
  • To confirm the role of a specific plasmid in AS-48 biosynthesis and activity.

Main Methods:

  • Isolation and characterization of Enterococcus faecalis S-48 mutants lacking inhibitory properties.
  • Plasmid DNA analysis to identify potential gene-carrying plasmids.
  • Conjugation experiments to transfer plasmids between E. faecalis strains.

Related Experiment Videos

  • Determination of Minimum Inhibitory Concentration (MIC) of AS-48.

    • Main Results:

    • Mutants lacking AS-48 and Bc-48 production were successfully isolated.
    • A 56-kilobase plasmid, designated pMB2, was identified as potentially harboring the AS-48 genes.
    • Transfer of pMB2 into a plasmid-free E. faecalis strain (OG1X) resulted in AS-48 production.
    • The transconjugant strain OG1X(pMB2) exhibited AS-48 production with an MIC comparable to the donor strain.

    Conclusions:

    • The 56-kilobase plasmid pMB2 contains the genetic determinants for AS-48 production in Enterococcus faecalis S-48.
    • Successful transfer of pMB2 confers AS-48 antibiotic activity to a recipient strain, validating its role in biosynthesis.