Application of enzyme-linked-immuno-electro-diffusion-assay (ELIEDA) in diagnosis of bilharziasis

The sensitivity of the technique of immunoelectrodiffusion has been enhanced by combining it with an enzyme method. The precipitated immuno-complexes are treated with antibodies conjugated with an enzyme specific for each class of immuno-globulins. This association realizes the ELIEDA and enable the class of immunoglobulins involved in the reactions to be determined. The technique allows the follow up and the qualitative evaluation of the bilharzial antibody in natural and experimental affections.