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Updated: Jun 6, 2026
Alignment of Visible-Light Optical Coherence Tomography Fibergrams with Confocal Images of the Same Mouse Retina
Published on: June 30, 2023
Improved visualization of Henle fiber layer by changing the measurement beam angle on optical coherence tomography
Tomohiro Otani1, Yumiko Yamaguchi, Shoji Kishi
1Department of Ophthalmology, Gunma University School of Medicine, Maebashi, Gunma, Japan. otanito@showa.gunma-u.ac.jp
Purpose:
The outer plexiform layer (OPL) includes the photoreceptor synapse layer and Henle fiber layer (HFL) in the macular area. We attempted to improve optical coherence tomography imaging of the HFL by changing the angle of the measurement beam.
Methods:
Thirteen normal eyes underwent spectral domain optical coherence tomography. To focus the angle of the measurement beam perpendicular to the obliquely oriented HFL, the beam in the optical coherence tomography system was aimed at the periphery of the pupil.
Results:
The cross-sectional image of the macular area was inclined right or left if the beam entered from the periphery of the pupil. The reflectivity of the OPL increased because of strong backscattering of light in the half of the macula that was tilted down in cross-sectional images. On the opposite side, the reflectivity of HFL (the outer two-thirds of the OPL) decreased compared with that of the outer nuclear layer, but the photoreceptor synapse layer (the inner one-third of the OPL) remained highly reflective. The mean thicknesses of the OPL that could be visualized 1.0 mm and 1.5 mm from the central fovea were 73.7 ± 7.0 μm and 64.1 ± 8.2 μm, respectively.
Conclusion:
Positioning the measurement beam perpendicular to HFL enhanced visualization performance.
