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Updated: Jan 30, 2026

Production of Double-stranded DNA Ministrings
Published on: February 29, 2016
A robust system for production of minicircle DNA vectors
Mark A Kay1, Cheng-Yi He, Zhi-Ying Chen
1Department of Pediatrics and Genetics, Stanford University School of Medicine, Stanford, California, USA. markay@stanford.edu
None:
Minicircle DNA vectors allow sustained transgene expression in quiescent cells and tissues. To improve minicircle production, we genetically modified Escherichia coli to construct a producer strain that stably expresses a set of inducible minicircle-assembly enzymes, ΦC31 integrase and I-SceI homing endonuclease. This bacterial strain produces purified minicircles in a time frame and quantity similar to those of routine plasmid DNA preparation, making it feasible to use minicircles in place of plasmids in mammalian transgene expression studies.
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