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Related Experiment Videos

Reevaluation of serum-plasma differences in total cholesterol concentration.

T Cloey1, P S Bachorik, D Becker

  • 1Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, Md.

JAMA
|May 23, 1990
PubMed
Summary
This summary is machine-generated.

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Cholesterol levels measured in disodium ethylenediaminetetraacetate (EDTA) plasma are lower than in serum. However, these values are highly correlated, allowing for easy conversion for accurate cholesterol screening and lipoprotein analysis.

Area of Science:

  • Clinical Chemistry
  • Biochemistry
  • Medical Diagnostics

Background:

  • Accurate cholesterol measurement is crucial for cardiovascular risk assessment.
  • Discrepancies exist between serum and plasma cholesterol levels, impacting clinical interpretation.
  • Existing guidelines provide a general conversion factor, but current collection methods may alter this bias.

Purpose of the Study:

  • To quantify the difference in total cholesterol levels between serum and disodium ethylenediaminetetraacetate (EDTA) plasma.
  • To assess the correlation between serum and EDTA plasma cholesterol measurements.
  • To evaluate the clinical implications for cholesterol screening and lipoprotein analysis.

Main Methods:

  • Total cholesterol was measured in both serum and EDTA plasma samples from healthy students and individuals with elevated cholesterol.

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  • Statistical analysis was performed to determine the correlation and percentage difference between the two sample types.
  • Comparison was made with existing National Cholesterol Education Program Guidelines.

    • Main Results:

    • Cholesterol concentrations in EDTA plasma were, on average, 4.7% lower than in serum.
    • A very high correlation (r = .994) was observed between serum and EDTA plasma cholesterol values.
    • The observed negative bias in EDTA plasma exceeded the previously cited 3% value.

    Conclusions:

    • Measurements in EDTA plasma can be reliably converted to equivalent serum concentrations due to high correlation.
    • The increased EDTA concentration in modern collection tubes likely contributes to the greater negative bias.
    • These findings necessitate a re-evaluation of current guidelines for interpreting cholesterol screening and lipoprotein analysis from EDTA plasma.