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Aptamer-based turn-on fluorescent four-branched quaternary ammonium pyrazine probe for selective thrombin detection

Shengyong Yan1, Rong Huang, Yangyang Zhou

  • 1College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Hubei, Wuhan, 430072, P. R. China.

Chemical Communications (Cambridge, England)
|November 25, 2010
PubMed

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...