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Updated: Jun 6, 2026
DNA Methylation: Bisulphite Modification and Analysis
Published on: October 21, 2011
Bisulfite-free analysis of 5MeC-binding proteins and locus-specific methylation density using a microparticle-based
Simon R Corrie1, Pavel Sova, Qinghua Feng
1The University of Queensland, Australian Institute for Bioengineering and Nanotechnology, Centre for Biomarker Research and Development, St Lucia, Brisbane, Australia.
Abstract:
DNA methylation analysis is emerging as a new technique with potential capabilities for early cancer detection. However, current state-of-the-art techniques are not easily translatable into routine clinical methods. Herein we describe a bead-based flow cytometry assay which combines DNA hybridization to microparticles with 5MeC-specific proteins/antibodies. These assays can be used to study the binding properties of current and emerging 5MeC-binding proteins and may also have potential in the measurement of 5MeC density in clinical samples for cancer detection.
