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Conjugated Proteins02:50

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Related Experiment Video

Updated: Jun 6, 2026

Concanavalin A-Based Sedimentation Assay to Measure Substrate Binding of Glucan Phosphatases
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Published on: December 23, 2022

Carbohydrate-functionalized oligothiophenes for concanavalin A recognition.

Sylvia Schmid1, Amaresh Mishra, Peter Bäuerle

  • 1Institute of Organic Chemistry II and Advanced Materials, University of Ulm, Albert-Einstein-Allee 11, 89081 Ulm, Germany. sylvia.schmid@uni-ulm.de

Chemical Communications (Cambridge, England)
|November 25, 2010
PubMed
Summary

Researchers synthesized fluorescent, carbohydrate-functionalized oligothiophene hybrids using Sonogashira coupling. Mannose derivatives showed specific binding with Concanavalin A, a model lectin.

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Published on: July 6, 2012

Area of Science:

  • Carbohydrate Chemistry
  • Organic Synthesis
  • Supramolecular Chemistry

Background:

  • Oligothiophenes are valuable conjugated materials with diverse applications.
  • Carbohydrate functionalization can impart specific biological recognition properties.
  • Developing efficient synthetic routes for such hybrid materials is crucial.

Purpose of the Study:

  • To develop a versatile synthesis for fluorescent carbohydrate-functionalized oligothiophene hybrids.
  • To investigate the carbohydrate-lectin binding interactions of the synthesized compounds.

Main Methods:

  • Sonogashira-type cross-coupling reactions were employed for the synthesis.
  • Characterization of the resulting oligothiophene hybrids was performed.
  • Binding studies with Concanavalin A were conducted using mannose derivatives.

Main Results:

  • Successful synthesis of fluorescent, carbohydrate-functionalized oligothiophene hybrids was achieved.
  • The mannose-functionalized oligothiophenes exhibited specific binding affinity.
  • Concanavalin A demonstrated selective interaction with the mannose derivatives.

Conclusions:

  • Sonogashira coupling provides a versatile route to novel fluorescent carbohydrate-oligothiophene hybrids.
  • These hybrids show potential for applications in carbohydrate recognition and sensing.
  • The specific binding of mannose derivatives to Concanavalin A highlights their utility in biological studies.