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Updated: Jun 6, 2026
Quantification of Lipid Abundance and Evaluation of Lipid Distribution in Caenorhabditis elegans by Nile Red and Oil Red O Staining
Published on: March 5, 2018
Rapid quantification of lipids in Acremonium chrysogenum using Oil red O
Hyun Yong Shin1, Jin Young Lee, Eun Ji Kim
1Department of Chemical and Biological Engineering, Korea University, 1-5Ka, Anam-Dong, Sungbuk-Ku, Seoul, 136-701, Republic of Korea.
Abstract:
A method based on staining condition and volume of culture broth for the rapid estimation of the level of intracellular lipids in Acremonium chrysogenum using Oil red O was developed. Lipids in A. chrysogenum were strongly stained by the modified Oil red O after treatment for 10 min at 75°C. The results of the study indicated that the Oil red O staining method developed here is useful for the quantification of 0.1-5 mg ml(-1) of lipids in A. chrysogenum.
