Sputter deposited bioceramic coatings: surface characterisation and initial protein adsorption studies using
1Nanotechnology and Integrated Bioengineering Centre (NIBEC), University of Ulster at Jordanstown, Newtownabbey, Co, Antrim, Northern Ireland, UK. ar.boyd@ulster.ac.uk
Journal of Materials Science. Materials in Medicine
|November 25, 2010
Summary
Surface-matrix assisted laser desorption ionization mass spectrometry (Surface-MALDI-MS) directly detected unique protein adsorption patterns on hybrid calcium phosphate/titanium dioxide bioceramics. This reveals insights into implant bioactivity influenced by protein interactions.
Area of Science:
- Biomaterials Science
- Surface Chemistry
- Mass Spectrometry
Background:
- Protein adsorption on calcium phosphate (Ca-P) bioceramics is crucial for hard tissue implant bioactivity.
- Understanding these protein interactions is key to developing effective implant materials.
Purpose of the Study:
- To investigate protein adsorption onto novel hybrid Ca-P/titanium dioxide surfaces.
- To evaluate Surface-MALDI-MS for direct protein detection on these bioceramic coatings.
Main Methods:
- Hybrid Ca-P/titanium dioxide surfaces were fabricated using radio frequency (RF) magnetron sputtering with in situ annealing (500–700°C).
- Surface characterization involved X-ray Diffraction (XRD) and X-ray Photoelectron Spectroscopy (XPS).
- Protein adsorption from foetal bovine serum (FBS) was analyzed using Surface-MALDI-MS.
Main Results:
- Coatings annealed at 700°C exhibited a hybrid Ca-P and titanium dioxide surface layer with an increased Ca/P ratio (2.0–2.26).
- Surface-MALDI-MS revealed distinct protein adsorption patterns, with unique peaks below 23.1 kDa.
- Adsorbed proteins were identified as growth factors and lipoproteins.
Conclusions:
- Surface-MALDI-MS is a valuable tool for studying dynamic protein adsorption on bioceramic surfaces.
- The observed protein adsorption patterns likely influence the bioactivity of Ca-P/titanium dioxide coatings for hard tissue applications.
