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Supramolecular au nanoparticle assemblies as optical probes for enzyme-linked immunoassays

Roberto de la Rica1, Aldrik H Velders

  • 1Laboratory of Supramolecular Chemistry and Technology, MESA+ Research Institute for Nanotechnology, Faculty of Science and Technology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands. r.delarica@utwente.nl

Small (Weinheim an Der Bergstrasse, Germany)
|November 25, 2010
PubMed
Abstract

No abstract available in PubMed .

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Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.