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Suppressed primary osteoblast functions on nanoporous titania surface
Lingzhou Zhao1, Shenglin Mei, Wei Wang
1Department of Periodontology and Oral Medicine, School of Stomatology, The Fourth Military Medical University, Xi'an, China.
Journal of Biomedical Materials Research. Part A
|November 25, 2010
Summary
Titania nanotubes show potential for medical implants, but their biocompatibility is debated. This study found that while initial cell adhesion was unaffected, titania nanotextures impaired primary osteoblast growth and differentiation, potentially due to altered cell adhesion.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Cell Biology
Background:
- Titania nanotubes are promising for medical implants.
- Their tissue compatibility and effects on osteoblasts remain controversial.
- Primary osteoblasts offer a more relevant model for in vivo conditions than cell lines.
Purpose of the Study:
- To investigate the response of primary osteoblasts on anodized nanotextured titania surfaces.
- To compare the effects of different nanotexture morphologies (25 nm and 80 nm) on osteoblast behavior.
- To elucidate the mechanisms behind titania nanotube cytocompatibility.
Main Methods:
- Fabrication of titania nanotextured surfaces via anodization (5 V and 20 V).
- Culturing of primary osteoblasts on these surfaces.
- Assessment of cell adhesion, growth, differentiation (alkaline phosphatase activity, extracellular matrix deposition), and gene expression.
- Analysis of focal contact formation.
Main Results:
- Initial cell adhesion was not significantly affected by the nanotextured surfaces.
- Cell growth and differentiation markers (osteogenesis-related gene expression) were impaired on both nanotextured surfaces.
- Slightly increased intracellular alkaline phosphatase activity and extracellular matrix deposition were observed.
- Compromised focal contact formation was suggested as a potential cause for impaired cell behavior.
Conclusions:
- Anodized titania nanotextures may impede primary osteoblast growth and differentiation.
- Compromised focal adhesion formation could underlie the observed negative effects.
- Differences in primary osteoblast and osteoblastic cell line responses may explain the controversy in titania nanotube biocompatibility.
