Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Site-Targeted Drug Delivery Systems: Polymeric Carriers01:24

Site-Targeted Drug Delivery Systems: Polymeric Carriers

Polymeric carriers enhance targeted drug delivery by increasing efficacy while minimizing off-target effects. These carriers comprise a biodegradable polymeric backbone integrated with functional elements that enable targeting, improve physicochemical properties, and regulate drug release.Targeting MechanismsThe targeting ability of polymeric carriers is mediated by a homing device, which is a molecular recognition component designed to selectively bind to specific tissues or cells. Monoclonal...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multiomic data integration unveils molecular mechanisms underlying chestnut and skin tissue differentiation in Dezhou donkeys.

BMC genomics·2026
Same author

Liquid biopsy-based multi-omics approach for early detection of hepatocellular carcinoma (ASCEND-Hep): a multiphase prospective development and validation study.

BMC medicine·2026
Same author

DCAF7 enhances atherosclerosis in vascular endothelial cells by promoting SLC40A1 transcription through facilitating TNF-mediated NF-κB function.

Biochemistry and biophysics reports·2026
Same author

Ancestral DOF27 and GDH1 haplotypes drive rice adaptation to ammonium habitats.

Current biology : CB·2026
Same author

FK506-binding proteins OsFKBP57 and OsFKBP73 confer salt tolerance in rice by activating the SOS pathway while reducing grain protein accumulation through inhibition of BCAT activity.

Plant communications·2026
Same author

Mannose-binding lectin 2 secreted by hepatocellular carcinoma cells recruits and activates natural killer cells to reshape an immune-activated microenvironment.

PLoS biology·2026

Related Experiment Video

Updated: Jun 6, 2026

3D Printing of In Vitro Hydrogel Microcarriers by Alternating Viscous-Inertial Force Jetting
05:32

3D Printing of In Vitro Hydrogel Microcarriers by Alternating Viscous-Inertial Force Jetting

Published on: April 21, 2021

Genipin-crosslinked microcarriers mediating hepatocellular aggregates formation and functionalities.

Ting Ting Lau1, Chunming Wang, Sze Wei Png

  • 1Division of Bioengineering, School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore, Singapore.

Journal of Biomedical Materials Research. Part A
|November 25, 2010
PubMed
Summary

Genipin-crosslinked gelatin microspheres effectively support liver cell growth and aggregation. Larger microspheres (200-300 μm) show enhanced performance for liver cell delivery and functionality.

More Related Videos

Recombinant Collagen I Peptide Microcarriers for Cell Expansion and Their Potential Use As Cell Delivery System in a Bioreactor Model
08:43

Recombinant Collagen I Peptide Microcarriers for Cell Expansion and Their Potential Use As Cell Delivery System in a Bioreactor Model

Published on: February 7, 2018

Gene Transfection toward Spheroid Cells on Micropatterned Culture Plates for Genetically-modified Cell Transplantation
07:40

Gene Transfection toward Spheroid Cells on Micropatterned Culture Plates for Genetically-modified Cell Transplantation

Published on: July 31, 2015

Related Experiment Videos

Last Updated: Jun 6, 2026

3D Printing of In Vitro Hydrogel Microcarriers by Alternating Viscous-Inertial Force Jetting
05:32

3D Printing of In Vitro Hydrogel Microcarriers by Alternating Viscous-Inertial Force Jetting

Published on: April 21, 2021

Recombinant Collagen I Peptide Microcarriers for Cell Expansion and Their Potential Use As Cell Delivery System in a Bioreactor Model
08:43

Recombinant Collagen I Peptide Microcarriers for Cell Expansion and Their Potential Use As Cell Delivery System in a Bioreactor Model

Published on: February 7, 2018

Gene Transfection toward Spheroid Cells on Micropatterned Culture Plates for Genetically-modified Cell Transplantation
07:40

Gene Transfection toward Spheroid Cells on Micropatterned Culture Plates for Genetically-modified Cell Transplantation

Published on: July 31, 2015

Area of Science:

  • Biomaterials science
  • Regenerative medicine
  • Hepatology

Background:

  • Hepatocytes require solid anchors for survival and proliferation in liver regenerative medicine.
  • Microspherical cell carriers offer both 2D surfaces for initial cell attachment and 3D matrices for aggregate formation.
  • Genipin-crosslinked gelatin microspheres are investigated as potential scaffolds for liver cells.

Purpose of the Study:

  • To evaluate the efficacy of genipin-crosslinked gelatin microspheres as cell carriers for hepatocytes.
  • To compare the performance of different microsphere sizes (200-300 μm vs. smaller) for liver cell culture.
  • To assess hepatocellular aggregate formation and liver-specific functionalities on these microcarriers.

Main Methods:

  • Culture of human liver carcinoma cell line (HepG2) cells on genipin-crosslinked gelatin microspheres of two distinct sizes.
  • Assessment of cell adhesion, proliferation, and spontaneous aggregate formation.
  • Evaluation of liver-specific functionalities on the cultured cell aggregates.

Main Results:

  • Both smaller and larger genipin-crosslinked gelatin microspheres supported HepG2 cell adhesion and proliferation.
  • Spontaneous formation of hepatocellular aggregates was observed on both types of microcarriers.
  • Larger microspheres (200-300 μm) demonstrated more favorable aggregate formation and enhanced liver-specific functionalities compared to smaller ones.

Conclusions:

  • Genipin-crosslinked gelatin microspheres serve as competent vehicles for liver cell delivery.
  • Microsphere size is a critical factor influencing hepatocellular aggregate formation and functionality.
  • These findings support the potential of optimized genipin-crosslinked microcarriers in liver regenerative medicine.