Related Experiment Videos
Sequence-specific [1H]NMR resonance assignments and secondary structure identification for 1- and 2-zinc finger
D Neuhaus1, Y Nakaseko, K Nagai
1MRC Laboratory for Molecular Biology, Cambridge, England.
FEBS Letters
|March 26, 1990
Abstract:
Complete [1H]NMR resonance assignments are presented for the second of the three zinc fingers from SW15 as it appears in both 1- and 2-finger constructs. Signals from finger 2 are unaffected by the presence or absence of finger 1, showing that the protein has a modular construction. The structure of finger 2 comprises a helix running from N56 to Q67, and a region approximating to an anti-parallel beta-sheet running from Y42 to F53. These features combine to produce a hydrophobic core in the structure involving the invariant residues Y42, F53, L59 and H62.