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Related Experiment Video

Updated: Jun 6, 2026

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples
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Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

Published on: May 4, 2012

Multiplex suspension array for human anti-carbohydrate antibody profiling.

Tatiana Pochechueva1, Alexander Chinarev, Marianne Spengler

  • 1University Hospital Zürich, Translational Research Group, Gynecological Research Department, Frauenklinikstrasse 10, Nord I D222, CH-8091, Zurich, Switzerland. Tatiana.Pochechueva@usz.ch

The Analyst
|November 26, 2010
PubMed
Summary
This summary is machine-generated.

A new microsphere-based assay enables multiplexed detection of glycan-binding antibodies. This sensitive method accurately identifies antibodies against ABO blood group antigens, offering a promising tool for immunological studies.

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Area of Science:

  • Immunology
  • Biotechnology
  • Analytical Chemistry

Background:

  • Glycan-binding antibodies are crucial in immune responses, impacting cancer, autoimmune diseases, and neurological disorders.
  • Accurate detection of these antibodies is vital for understanding and diagnosing various conditions.

Purpose of the Study:

  • To develop a novel microsphere-based flow-cytometric immunoassay for multiplexed detection of glycan-binding antibodies.
  • To evaluate the efficiency and accuracy of this assay for detecting antibodies against ABO blood group antigens.

Main Methods:

  • A suspension array (microsphere-based flow cytometry) was employed for assay development.
  • Glycoconjugates were immobilized onto fluorescent microspheres using end-biotinylated glycopolymers.
  • The assay was validated for multiplexed detection of IgM and IgG antibodies against ABO-related glycans.

Main Results:

  • The optimized method demonstrated high sensitivity and required minimal glycan amounts.
  • Multiplexed detection of anti-glycan IgM and IgG antibodies showed excellent correlation with singleplex assays (r = 0.95-0.99).
  • Singleplex assay performance correlated well with standard ELISA (r > 0.94).

Conclusions:

  • The developed microsphere-based assay is a rapid, sensitive, and reproducible method for detecting anti-glycan antibodies.
  • This multiplexed approach holds significant promise for immunological research and diagnostics.
  • The assay's ability to detect antibodies against ABO blood group antigens was effectively demonstrated.