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A simple reverse genetics approach to elucidating the biosynthetic pathway of nocathiacin
Maochen Wei1, Jing Deng, Shuzhen Wang
1Laboratory of Chemical Biology, China Pharmaceutical University, 24 Tongjia Street, Nanjing, Jiangsu Province, 210009, People's Republic of China.
Abstract:
Genomic library screening and genome mining are currently employed to identify biosynthetic gene clusters of thiopeptides. To elucidate the biosynthetic pathway of nocathiacin, we present a new approach with the application of simple reverse genetics. A relationship between structural features of thiopeptides and their biosynthetic pathways is established and is a starting point for speedily elucidating biosynthetic genes of various ribosomally-synthesized bioactive peptides with diverse modifications.
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