Preparation of low-molecular-mass peptides by enzyme autolysis oF Paphia undulata

Paphia undulata was used to prepare low-molecular-mass peptides by enzyme autolysis. The optimum condition for autolysis was studied to reach a maximum yield of peptides. Results showed that the yield of low-molecular-mass peptides was 4.33% and 78.57% of the total protein can be recovered under the optimized condition: pH = 6.5, temperature = 45 °C, reaction time = 4 h, ratio of starting material and water = 1:3 and the enzyme dosage was 1374.0 U/g.