Related Experiment Video
Updated: Jun 6, 2026

Estimating the Yield of Compounds on the TLC Plate via the Blue-LED Illumination Technique
Published on: October 6, 2022
Stability-indicating TLC-densitometric method for estimation of dexrabeprazole and domperidone in pharmaceutical
Sohan S Chitlange1, Amir I Mulla, Ganesh R Pawbake
1Padmashri Dr. D.Y. Patil Institute of Pharmceutical Sciences and Research, Sant Tukaram Nagar, Pimpri, Pune, Maharashtra, India. sohanchitlange@rediffmail.com
Abstract:
A stability-indicating thin layer chromatographic (TLC) method for determination of dexrabeprazole (DEX) and domperidone (DOM) in combined dosage form has been developed and validated. The mobile phase selected was acetone:toluene:methanol (4.5:4.5:0.5, v/v/v) with ultraviolet (UV) detection at 285 nm. The retention factors for DEX and DOM were found to be 0.49 ± 0.02 and 0.24 ± 0.03, respectively. The method was validated with respect to linearity, accuracy, precision, and robustness. The linearity range for DEX was 50-350 ng/band (r² = .9960) and for DOM was 100-700-n /band (r² = .9982), respectively. The method was successfully applied for the analysis of drugs in pharmaceutical formulation.
More Related Videos
Related Concept Videos
Therapeutic Drug Monitoring: Drug Analysis Methods
Drug Dissolution: Requirements and Profile Comparison
Protein-Drug Binding: Determination Methods
Indirect methods involve isolating the bound drug from its free form in biological samples such as blood, serum, or plasma. These techniques aim to measure the percentage of drugs bound to proteins. Equilibrium dialysis is a commonly used method where the free drug concentration at equilibrium is measured by separating the bound...
Clinically Relevant Drug Product Specifications: Methods of Establishment
Determination of Multiple Dosing Parameters: Loading and Maintenance Doses
Measurement of Bioavailability: Pharmacodynamic Methods

