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Respiratory viruses in children admitted to hospital intensive care units: evaluating the CLART® Pneumovir DNA array

Emilie Frobert1, V Escuret, E Javouhey

  • 1Laboratoire de Virologie Est, Centre de Biologie et Pathologie Est, Hospices Civils de Lyon, Bron Cedex, Lyon, France. emilie.frobert@chu-lyon.fr

Journal of Medical Virology
|November 26, 2010
PubMed

Insights

This study found that viruses are common in pediatric intensive care unit (PICU) patients, with respiratory syncytial virus (RSV) and rhinovirus frequently detected. The CLART® Pneumovir DNA array effectively identified multiple viral infections in critically ill children.

Area of Science:

  • Pediatric Infectious Diseases
  • Virology
  • Critical Care Medicine

Background:

  • Viral respiratory infections are a major cause of severe illness and hospitalization in children.
  • Accurate and rapid viral detection is crucial for managing pediatric respiratory distress in intensive care settings.

Purpose of the Study:

  • To investigate the spectrum of respiratory viruses in children admitted to a hospital intensive care unit (ICU).
  • To evaluate the utility of the CLART® Pneumovir DNA array assay for simultaneous detection of multiple respiratory viruses.

Main Methods:

  • Respiratory specimens from 53 children under 2 years old admitted to the ICU during the 2008-2009 winter season were analyzed.
  • The CLART® Pneumovir DNA array assay was used to detect 11 genera of respiratory viruses.
  • Virological findings were correlated with clinical features, risk factors, and severity criteria.

Main Results:

  • At least one virus was detected in 78% of samples.
  • Respiratory syncytial virus (RSV) was identified in 73.6% and rhinovirus in 24.6% of positive samples.
  • Multiple infections were more common in children without risk factors (42%).
  • RSV appeared to cause severe symptoms independently, as intubation needs did not differ between single and co-infections.

Conclusions:

  • The CLART® Pneumovir DNA array is effective for diagnosing severe viral respiratory infections in critically ill children.
  • This assay can identify a broader range of viruses than conventional methods, especially in complex ICU cases.
  • Understanding viral co-infections is important for managing pediatric respiratory distress.

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