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[Cloning of hnRNP A2/B1 gene and detection of its expression in lung cancer tissues]
1Department of Respiratory, Southwest Hospital, Third Military Medical University, Chongqing 400038, P.R.China.
Background:
There is no any specific dynamic criterion in early diagnosis of lung cancer yet. The aim of this study is to obtain the cDNA sequence of hnRNP A2/B1 and then determine its expression in patients with lung cancer, and to provide experimental data for the early diagnosis and treatment of lung cancer.
Methods:
Total RNA was isolated from A549 cells and RT-PCR was performed. The fragment was cloned into PUCm-T plasmids and further sequenced. hnRNP A2/B1 expression was detected in 41 patients with lung cancer and 13 patients with carcinoma-free diseases by in situ hybridization.
Results:
The fragment was identified by DNA sequencing. The positive rate of hnRNP A2/B1 was 87.8% (36/41) in lung cancer tissues, which was significantly higher than that in control group (23.1%, 3/13) (P < 0.01). hnRNP A2/B1 was localized in the cytoplasm and/or nucleus, but mainly in the cytoplasm. Four subtypes of lung cancer all showed positive staining of hnRNP A2/B1 protein. The positive rate of hnRNP A2/B1 was 91.3% (21/23) in squamous cell carcinoma, and 78.6% (11/14) in adenocarcinoma (P > 0.05).
Conclusions:
hnRNP A2/B1 has been cloned successfully. It can highly express in lung cancer tissues, but it does not correlate with histological classification of lung cancer.
