Differential methylation of CpG islands within the dermo1 gene promoter in several cancer cell lines
Related Experiment Video
Updated: Jun 6, 2026

Methyl-binding DNA capture Sequencing for Patient Tissues
Published on: October 31, 2016
Yubin Mao1, Hween-Boon Toh, Zhijie Ding
1Medical College, Xiamen University, Xiamen, P.R. China.
Abstract:
Inactivation of tumor-related genes by promoter hypermethylation is a common epigenetic event in the development of variety of tumors. Dermo1 (also called twist2) is a novel cancer-related gene which belongs to the basic helix-loop-helix (bHLH) transcription factor family. Herein, we report that dermo1 expression was sporadically abrogated in human cancer cells by transcriptional silencing associated with CpG island promoter hypermethylation. Direct sequencing of bisulfite-modified DNA from a panel of seven human cancer cell lines (HL60, Molm14, MV4-11, RS4:11, MDM-BA231, H358, and H1299) revealed that CpG dinucleotides in the dermo1 promoter were methylated. RT-PCR results demonstrated that dermo1 CpG island hypermethylation was accompanied by a low basal dermo1 expression level. Our data implicate dermo1 as a tumor suppressor gene and a valuable molecular marker for human cancer.
Related Concept Videos
Epigenetic Regulation
X-chromosome...
Epigenetic Regulation
Induced Pluripotent Stem Cells
Somatic cells are...
Abnormal Proliferation
Genomic Imprinting and Inheritance
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...

