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The influence of oxyhemoglobin affinity on tissue oxygen consumption
Annals of Surgery
|February 1, 1976
Summary
Altering hemoglobin
Area of Science:
- Physiology
- Biochemistry
Background:
- Evaluating hemoglobin's oxygen affinity is complex in vivo due to multiple interacting factors.
- Previous studies have not isolated hemoglobin-oxygen affinity as the sole variable affecting oxygen delivery.
Purpose of the Study:
- To investigate the impact of hemoglobin-oxygen affinity on tissue oxygen consumption.
- To isolate hemoglobin-oxygen affinity as the sole variable in oxygen delivery using an isolated perfused canine hindlimb model.
Main Methods:
- Established a membrane lung system for precise control of blood flow, temperature, arterial oxygen content, and pH.
- Utilized parallel perfusion systems with isolated canine hindlimbs.
- Perfused hindlimbs with autologous stored (left-shifted) and fresh (right-shifted) blood.
Main Results:
- Demonstrated significant differences in 2,3-DPG concentrations, P50 values, and oxygen consumption between the two perfusion systems.
- Observed that decreased hemoglobin affinity for oxygen correlated with increased oxygen off-loading at the tissue level.
Conclusions:
- Reduced hemoglobin-oxygen affinity facilitates greater oxygen release to tissues.
- This study successfully isolated hemoglobin-oxygen affinity to clarify its role in oxygen delivery and consumption.