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Regulation of microRNA-145 by growth arrest and differentiation

Gaspare La Rocca1, Bin Shi, Alessandra Audia

  • 1Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA 19107, USA.

Experimental Cell Research
|November 30, 2010
PubMed

Insights

MicroRNA145 (miR145), a tumor suppressor, increases in differentiating and growth-arrested cells. Its expression may be required early or late in cell differentiation.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • MicroRNA145 (miR145) functions as a tumor suppressor.
  • miR145 is known to inhibit cancer cell growth, induce differentiation, and promote apoptosis, leading to growth arrest.

Purpose of the Study:

  • To investigate the functional effects of miR145.
  • To clarify the expression patterns of miR145 in various cellular conditions and cell lines.

Main Methods:

  • Expression analysis of miR145.
  • Investigation across diverse cell lines and conditions.

Main Results:

  • miR145 levels were observed to increase in differentiating cells.
  • miR145 expression also increased in growth-arrested cells, independent of differentiation.
  • Increased miR145 expression during differentiation can occur as a late event.

Conclusions:

  • miR145 expression is linked to cell differentiation and growth arrest.
  • miR145 may play a role at various stages of the differentiation process.

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