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Structural modifications induced by the mtDBP-C protein in the replication origin of Xenopus laevis mitochondrial DNA
B Mignotte1, B Theveny, B Revet
1Laboratoire de Biologie Générale, Université de Paris Sud, Orsay, France.
Biochimie
|January 1, 1990
Abstract:
The structure of the non-coding region of Xenopus laevis mitochondrial DNA has been studied by electron microscopy analysis of DNA molecules end-labelled with streptavidin-ferritin. We have shown that the effect of a protein modifying the shape of the DNA double-helix can be studied and precisely located by this method. It was found that the non-coding region contains curved segments and that the mitochondrial protein mtDBP-C preferentially enhances the curvature of the promoters-replication origin region.