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Characterization of DOPA decarboxylase mRNA in rat pheochromocytoma
G Bruneau1, M Krieger-Poullet, F Coge
1Laboratoire de Biochimie Cellulaire, Collège de France, Paris.
Biochimie
|January 1, 1990
Abstract:
Total poly (A+) RNA has been extracted from rat pheochromocytoma and translated in vitro by means of a reticulocyte lysate system. We show that two antisera, prepared against pig kidney DOPA decarboxylase (DDC) or rat pheochromocytoma DDC, immunoprecipitate an in vitro synthetized 50 kDa polypeptide identified as DDC by competition experiments with pure DDC. The proportion of specific mRNA has been calculated and represents 0.05% of total poly A+ mRNA. Its size has been established by electrophoresis in methylmercuric hydroxide containing agarose gel, corresponding to a 2.2 kb length mRNA.