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Updated: Jun 6, 2026

Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases
22:00

Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases

Published on: November 21, 2010

Crystallizing membrane proteins for structure determination using lipidic mesophases.

Martin Caffrey1, Christopher Porter

  • 1Membrane Structural and Functional Biology Group, Schools of Biochemistry and Immunology and Medicine, Trinity College Dublin. martin.caffrey@tcd.ie

Journal of Visualized Experiments : Jove
|November 30, 2010
PubMed
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This study details a versatile lipidic cubic phase (LCP) method for crystallizing membrane proteins. The in meso technique enables X-ray crystallographic structure determination for diverse protein types, including G protein-coupled receptors.

Area of Science:

  • Structural Biology
  • Biochemistry
  • Membrane Protein Crystallization

Background:

  • Membrane proteins are crucial for cellular functions but challenging to crystallize.
  • The lipidic cubic phase (LCP) or in meso method offers a promising approach for membrane protein crystallization.
  • Previous studies have demonstrated the utility of LCP for various protein structures.

Purpose of the Study:

  • To provide a detailed protocol for crystallizing membrane proteins using the in meso method.
  • To highlight the versatility of the LCP technique for diverse protein targets.
  • To facilitate the structure determination of challenging membrane proteins.

Main Methods:

  • Reconstitution of membrane proteins into a monoolein-based lipidic mesophase.

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Use of a Robot for High-throughput Crystallization of Membrane Proteins in Lipidic Mesophases

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Last Updated: Jun 6, 2026

Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases
22:00

Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases

Published on: November 21, 2010

Crystallization of Membrane Proteins in Lipidic Mesophases
11:53

Crystallization of Membrane Proteins in Lipidic Mesophases

Published on: March 28, 2011

Use of a Robot for High-throughput Crystallization of Membrane Proteins in Lipidic Mesophases
20:21

Use of a Robot for High-throughput Crystallization of Membrane Proteins in Lipidic Mesophases

Published on: September 1, 2012

  • Manual setup of crystallization plates using the in meso technique.
  • Utilizing X-ray crystallography for structure determination.

    • Main Results:

    • The in meso method has been successfully applied to crystallize various membrane proteins, including prokaryotic and eukaryotic examples.
    • Structures of monomeric, multimeric, chromophore-containing, and chromophore-free proteins have been solved.
    • Recent successes include crystallization of human engineered beta2-adrenergic and adenosine A2a G protein-coupled receptors.
    • The preparation of protein-loaded mesophase and manual crystallization setup takes approximately one hour.

    Conclusions:

    • The lipidic cubic phase (in meso) method is a versatile and efficient technique for membrane protein crystallization.
    • This protocol enables the structural elucidation of a wide range of membrane proteins, including important therapeutic targets like GPCRs.
    • Further video articles will cover additional steps such as crystal harvesting.