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Related Experiment Video

Updated: Jun 6, 2026

Dynamic Multiparameter Platelet Function Assessment Using a Capacitive Biosensor
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Dynamic Multiparameter Platelet Function Assessment Using a Capacitive Biosensor

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A New Method for Measuring the Dynamic Shape Change of Platelets.

Max-Joseph Kraus1, Erwin F Strasser, Reinhold Eckstein

  • 1Department of Transfusion Medicine and Hemostasis, University Hospital Erlangen, Germany.

Transfusion Medicine and Hemotherapy : Offizielles Organ Der Deutschen Gesellschaft Fur Transfusionsmedizin Und Immunhamatologie
|November 30, 2010
PubMed
Summary

This study introduces a new method for measuring platelet shape changes, revealing dynamic alterations in platelet size and filopodia structure. The findings enable precise analysis of platelet morphology and surface features.

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Area of Science:

  • Hematology
  • Cell Biology
  • Microscopy

Background:

  • Platelet shape change is a complex, dynamic process with various classifications.
  • Accurate measurement of platelet structure requires advanced methodologies.
  • Existing methods may not fully capture the nuances of platelet shape dynamics.

Purpose of the Study:

  • To develop and validate an improved method for measuring platelet shape.
  • To document dynamic changes in platelet morphology and filopodia structure.
  • To enable exact analysis of platelet size and surface features.

Main Methods:

  • Utilized dark field light microscopy with a CMOS camera to capture images of platelet-rich plasma (PRP).
  • Employed a self-developed ImageJ plugin for precise measurement of platelet diameter and filopodia length.

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Last Updated: Jun 6, 2026

Dynamic Multiparameter Platelet Function Assessment Using a Capacitive Biosensor
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A Uniform Shear Assay for Human Platelet and Cell Surface Receptors via Cone-plate Viscometry
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Live-cell Imaging of Platelet Degranulation and Secretion Under Flow

Published on: July 10, 2017

  • Analyzed photographic snapshots taken at 5 and 30 minutes post-preparation.

    • Main Results:

    • Observed a significant swelling of the granulomere (2.06 to 2.33 μm, p < 0.05).
    • Documented a reduction in pseudopodia (2.10 to 1.78 μm, p < 0.05) concurrent with hyalomer diameter increase (3.29 to 3.50 μm, p < 0.05).
    • Noted a significant increase in pseudopodia length (2.68 to 3.67 μm, p < 0.005) alongside hyalomer diameter growth (6.58 to 7.94 μm, p < 0.05).

    Conclusions:

    • The study successfully revealed and documented dynamic changes in platelet size and filopodia structure.
    • The developed method provides an exact analysis of platelet size and surface structures.
    • This technique enhances the understanding of platelet morphology in platelet-rich plasma.