Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 6, 2026

Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations
09:07

Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations

Published on: August 22, 2019

Screening Donors for Rare Antigen Constellations.

Franz F Wagner1

  • 1DRK-Blutspendedienst NSTOB, Zentralinstitut Springe, Germany.

Transfusion Medicine and Hemotherapy : Offizielles Organ Der Deutschen Gesellschaft Fur Transfusionsmedizin Und Immunhamatologie
|November 30, 2010
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

International Society of Blood Transfusion Working Party on Red Cell Immunogenetics and Blood Group Terminology Report of Gothenburg, Barcelona and four virtual business meetings: Update on blood group systems.

Vox sanguinis·2025
Same author

Monitoring the SARS-CoV-2 Pandemic: Prevalence of Antibodies in a Large, Repetitive Cross-Sectional Study of Blood Donors in Germany-Results from the SeBluCo Study 2020-2022.

Pathogens (Basel, Switzerland)·2023
Same author

New ultraviolet C light-based method for pathogen inactivation of red blood cell units.

Transfusion·2022
Same author

Blood Groups and Their Correlation with Hereditary Disease.

Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie·2022
Same author

NG_007494.1(RHD):c.[4A>T;5G>C;6_7insG] with an RhD-negative phenotype.

Transfusion·2020
Same author

Two novel antithetical KN blood group antigens may contribute to more than a quarter of all KN antisera in Europe.

Transfusion·2020

Simple polymerase chain reaction (PCR) methods are now used to screen blood donors for rare antigen constellations. These techniques efficiently identify Dombrock antigens and high-frequency antigen-negative donors, improving blood transfusion safety.

Area of Science:

  • Transfusion Medicine
  • Immunology
  • Molecular Biology

Background:

  • Screening blood donors for specific red blood cell antigens is crucial for preventing transfusion reactions.
  • Rare antigen constellations and high-frequency antigens pose challenges in blood donor selection.
  • Traditional serological methods can be time-consuming and labor-intensive.

Purpose of the Study:

  • To evaluate the implementation and effectiveness of simple polymerase chain reaction (PCR) methods for screening blood donors.
  • To assess the utility of PCR with enzyme digestion for Dombrock antigen typing.
  • To identify donors negative for high-frequency antigens using PCR with sequence-specific priming.

Main Methods:

  • Polymerase chain reaction (PCR) with enzyme digestion was employed for Dombrock antigen typing.

More Related Videos

Identification of Rare Antigen-Specific T Cells from Mouse Lungs with Peptide:Major Histocompatibility Complex Tetramers
09:15

Identification of Rare Antigen-Specific T Cells from Mouse Lungs with Peptide:Major Histocompatibility Complex Tetramers

Published on: July 19, 2024

Personalized Peptide Arrays for Detection of HLA Alloantibodies in Organ Transplantation
08:07

Personalized Peptide Arrays for Detection of HLA Alloantibodies in Organ Transplantation

Published on: September 6, 2017

Related Experiment Videos

Last Updated: Jun 6, 2026

Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations
09:07

Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations

Published on: August 22, 2019

Identification of Rare Antigen-Specific T Cells from Mouse Lungs with Peptide:Major Histocompatibility Complex Tetramers
09:15

Identification of Rare Antigen-Specific T Cells from Mouse Lungs with Peptide:Major Histocompatibility Complex Tetramers

Published on: July 19, 2024

Personalized Peptide Arrays for Detection of HLA Alloantibodies in Organ Transplantation
08:07

Personalized Peptide Arrays for Detection of HLA Alloantibodies in Organ Transplantation

Published on: September 6, 2017

  • PCR with sequence-specific priming was utilized to detect high-frequency antigen-negative donors.
  • The advantages and disadvantages of these PCR-based methods were discussed.

    • Main Results:

    • Simple PCR methods have been successfully implemented for screening blood donors.
    • PCR techniques allow for efficient typing of donor cohorts for specific antigens.
    • These methods facilitate the identification of donors with rare antigen profiles.

    Conclusions:

    • Simple PCR methods offer a viable and efficient approach for screening blood donors for rare antigen constellations.
    • These molecular techniques enhance the ability to identify suitable donors, thereby improving blood transfusion safety.
    • The current state and future potential of PCR-based donor screening are promising.