Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...
Combinatorial Gene Control02:33

Combinatorial Gene Control

Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ovarian Cancer Susceptibility and Chemosensitivity to KRAS Modulation.

International journal of molecular sciences·2026
Same author

Nonglycosylated, Legumain-Cleavable ISACs Drive Potent Antitumor Immunotherapy via a Bystander Effect.

Molecular cancer therapeutics·2025
Same author

Correction to "DNA G-Quadruplex and i-Motif Structure Formation Is Interdependent in Human Cells".

Journal of the American Chemical Society·2025
Same author

Dissecting the Efficacy and Immunogenicity of TLR7 Agonist-Antibody Conjugates through the Lens of Fc Effector Function, Conjugation Strategies, and Linker Cleavability.

Journal of medicinal chemistry·2025
Same author

Legumain-Cleavable Top1i Antibody-Drug Conjugates without Self-Immolative Spacers Demonstrate Potent Antitumor Activity.

Journal of medicinal chemistry·2025
Same author

Resin-Supported Site-Specific Antibody Conjugation Method Leads to Antibody-Drug Conjugates with Retained Efficacy and Improved Stability.

Bioconjugate chemistry·2025

Related Experiment Video

Updated: Jun 6, 2026

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

Targeting MYC Expression through G-Quadruplexes.

Tracy A Brooks1, Laurence H Hurley

  • 1College of Pharmacy, BIO5 Institute, and Arizona Cancer Center, University of Arizona, Tucson, AZ, USA.

Genes & Cancer
|November 30, 2010
PubMed
Summary

Scientists discovered a novel mechanism controlling MYC gene expression using G-quadruplex DNA structures. Targeting these structures with small molecules offers a new therapeutic strategy for gene regulation.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • MYC gene expression is crucial for cell growth and is often dysregulated in cancer.
  • G-quadruplexes are four-stranded DNA structures with emerging roles in biological processes.
  • The nuclease hypersensitive element III(1) (NHE III(1)) upstream of the MYC P1 promoter is involved in transcriptional regulation.

Purpose of the Study:

  • To elucidate a novel mechanism for MYC expression control involving G-quadruplex DNA structures.
  • To explore the role of specific proteins (NM23-H2 and nucleolin) in regulating G-quadruplex formation and MYC expression.
  • To investigate the therapeutic potential of targeting G-quadruplexes with small molecules.

Main Methods:

  • Review of existing literature on G-quadruplexes and MYC gene regulation.

More Related Videos

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
08:28

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
03:38

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

Related Experiment Videos

Last Updated: Jun 6, 2026

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
08:28

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
03:38

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

  • Analysis of the structural dynamics of the NHE III(1) region under transcriptional stress.
  • Examination of protein-DNA interactions involving NM23-H2 and nucleolin with G-quadruplex structures.

    • Main Results:

    • Transcription-induced negative superhelicity converts duplex DNA in the NHE III(1) region to G-quadruplex and i-motif structures, silencing MYC expression.
    • NM23-H2 resolves G-quadruplexes to activate MYC expression, while nucleolin folds them to silence it.
    • Inhibition of NM23-H2 or redistribution of nucleolin can modulate MYC expression.
    • Quarfloxin, a G-quadruplex-interactive compound, acts by redistributing nucleolin.

    Conclusions:

    • G-quadruplex structures in the MYC promoter region represent a novel regulatory mechanism for gene expression.
    • Proteins like NM23-H2 and nucleolin play critical roles in modulating MYC expression via G-quadruplex dynamics.
    • Targeting G-quadruplexes with small molecules, such as Quarfloxin, offers a promising therapeutic avenue for controlling MYC expression.