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Updated: Jun 6, 2026

08:37
Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
Published on: March 30, 2015
A novel real-time PCR method based on signaling-by-incorporation
Da-Rae Kim1, Hee-Chul Ahn, Won-Ja Lee
1Biomedical Research Center, Korea Institute of Science and Technology, PO Box 131, Cheongryang, Seoul 130-650, South Korea.
Abstract:
We describe a novel real-time PCR method, which utilizes the in situ generated signal upon incorporation of a fluorogenic nucleotide. The method has been compared with a conventional method and evaluated for practical uses such as quantification of cDNA and malaria diagnosis.
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Real Time RT-PCR
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
The real-time quantification of the number of amplified products is...
PCR - Polymerase Chain Reaction
Overview

