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The Terroir Concept Interpreted through Grape Berry Metabolomics and Transcriptomics
Published on: October 5, 2016
iTRAQ-based profiling of grape berry exocarp proteins during ripening using a parallel mass spectrometric method
Maria José Martínez-Esteso1, Juan Casado-Vela, Susana Sellés-Marchart
1Grupo de Proteómica y Genómica Funcional de Plantas, Dept. Agroquímica y Bioquímica, Facultad de Ciencias, Universidad de Alicante, Alicante, Spain.
Molecular Biosystems
|November 30, 2010
Summary
This study used iTRAQ to profile grapevine berry ripening proteins, identifying key candidates for quality and disease resistance. The method enhanced spectral identification, revealing deregulated pathways during ripening.
Area of Science:
- Proteomics
- Plant Biology
- Biochemistry
Background:
- Grapevine berry ripening involves complex molecular changes.
- Understanding protein dynamics is crucial for berry quality and development.
Purpose of the Study:
- To analyze protein profiles during grapevine berry ripening using 4-plex iTRAQ.
- To identify proteins involved in berry quality, growth regulation, and disease resistance.
Main Methods:
- Utilized the 4-plex iTRAQ platform for protein profiling across four ripening stages.
- Employed mass spectrometry with parallel acquisition (CID/HCD) on an Orbitrap instrument.
- Searched spectra against a Vitis-specific protein database, including whole genome sequences.
Main Results:
- Identified 695 unique proteins and quantified 513.
- Achieved a 5-fold increase in spectra suitable for peptide identification.
- Discovered deregulated biological processes and metabolic pathways during ripening.
Conclusions:
- Identified potential candidate proteins of Vitis origin regulating berry quality, growth, and disease resistance.
- The applied proteomic approach effectively revealed molecular changes during ripening.
- Highlights proteins crucial for grapevine berry development and resilience.
