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Related Experiment Video

Updated: Jun 6, 2026

AAV Deployment of Enhancer-Based Expression Constructs In Vivo in Mouse Brain

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Published on: March 31, 2022

Efficient expression from one CMV enhancer controlling two core promoters.

Christina Rottbøll Andersen¹, Lars Søegaard Nielsen, Alexandra Baer

¹Symphogen A/S, Elektrovej Building 375, 2800, Lyngby, Denmark. cra@symphogen.com

Molecular Biotechnology

|November 30, 2010

Summary

Optimizing recombinant antibody production, this study found that a truncated human cytomegalovirus (CMV) promoter complex significantly boosted expression. Removing a specific upstream region led to a 12-fold increase in antibody yield in CHO cells.

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Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions

Published on: June 28, 2018

Area of Science:

Biotechnology
Molecular Biology
Genetic Engineering

Background:

The human cytomegalovirus (CMV) promoter/enhancer is widely used for high recombinant protein expression in mammalian cells, particularly for antibody production.
A common vector design utilizes a dual divergent promoter complex for antibody gene transcription.
Unexpectedly low expression was observed with a double identical CMV promoter complex.

Purpose of the Study:

To optimize recombinant antibody expression using modified CMV promoter complexes.
To investigate the impact of promoter truncation and enhancer-driven dual core promoter activity on expression levels.
To enhance antibody production in CHO DG44 cells.

Main Methods:

Construction and analysis of truncated CMV promoter complexes with a single enhancer driving two divergent minimal core promoters.
Evaluation of antibody expression via transient transfection.
Assessment of antibody expression following stable, site-specific integration into CHO DG44 cells.

Main Results:

One CMV enhancer could successfully drive transcription from two divergent minimal CMV core promoters.
Efficient expression from both divergent core promoters required the removal of a unique upstream region adjacent to the enhancer.
The optimized truncated promoter complex achieved a 12-fold increase in antibody expression compared to the full-length double CMV promoter after stable integration.

Conclusions:

Truncated CMV promoter complexes offer a superior strategy for enhancing recombinant antibody expression.
Removal of specific upstream regulatory elements is critical for maximizing the efficacy of dual divergent promoters.
This optimization significantly improves antibody production in stable CHO cell lines.