Quantitative analysis of a prostate-specific antigen in serum using fluorescence immunochromatography

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Immunotechnology

Background:

• Prostate-specific antigen (PSA) is a key biomarker for prostate cancer detection and monitoring.
• Accurate and sensitive quantification of PSA is crucial for effective clinical management.
• Existing methods may have limitations in speed, cost, or sensitivity.

Purpose of the Study:

• To develop and validate a quantitative fluorescence immunochromatographic assay for PSA detection in human blood serum.
• To establish the analytical performance characteristics of the developed assay.

Main Methods:

• Development of a fluorescence immunochromatographic analysis system utilizing anti-PSA monoclonal antibodies (mAbs).
• The system comprises detection solution with fluorescence conjugates, an immunochromatographic strip, and a laser fluorescence scanner.
• PSA quantification is based on the area ratio between the control and test lines on the strip.

Main Results:

• The developed system successfully quantified PSA in human blood serum samples.
• The assay demonstrated a linear relationship between the area ratio and PSA concentration within the range of 0.72 to 46.0 ng/mL.
• A low detection limit of 0.72 ng/mL was achieved under optimal conditions.

Conclusions:

• Fluorescence immunochromatography provides a viable and sensitive method for quantitative PSA analysis.
• The developed assay shows potential for routine clinical use in PSA monitoring.
• This technique offers a sensitive and quantitative approach for biomarker detection.